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KMID : 1007520110200041155
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Food Science and Biotechnology
2011 Volume.20 No. 4 p.1155 ~ p.1159
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In vitro Characterization Study of Bacillus mojavensis KJS-3 for a Potential Probiotic
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Kim Kang-Min
Jung Tae-Sung
Ok Seon
Ko Chan-Young
Kang Jae-Seon
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Abstract
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The identification and characterization of Bacillus mojavensis KJS-3 was performed by in vitro tests. A 16S rDNA sequence and phylogenetic tree demonstrated that this isolate belongs to the B. mojavensis group. B. mojavensis KJS-3 supplies nutrients by synthesizing several vitamins. B. mojavensis KJS-3 produces α-amylase and protease. B. mojavensis KJS-3 is cultured well under aerobic conditions without gas production. B. mojavensis KJS-3 allows for assimilation of cholesterol and bile salt hydrolase activity. Finally, adhesion experiments using Caco-2 cells revealed that the adherence of B. mojavensis KJS-3 to Caco-2 cells was approximately 51.2±8.14%.
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KEYWORD
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Bacillus mojavensis KJS-3, characterization, probiotic, industrial application
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